You are using a browser version with limited support for CSS. Please note there may be errors present which affect the content, and all legal disclaimers apply. Peripherally induced T regulatory (pTreg) cells are essential for mitigating inflammatory responses to these agents1–4. While RORγt+ antigen-presenting cells (RORγt-APCs) were shown to program gut microbiota-specific pTreg5–7, their definition remains incomplete, and the APC responsible for food tolerance has remained elusive. Single-cell analyses of freshly resected mesenteric lymph nodes from a human organ donor, as well as multiple specimens of human intestine and tonsil, reveal candidate tolDC with co-expression of PRDM16 and RORC and an extensive transcriptome shared with mice, highlighting an evolutionarily conserved role across species. Our findings suggest that a better understanding of how tolDC develop and how they regulate T cell responses to food and microbial antigens could offer new insights into developing therapeutic strategies for autoimmune and allergic diseases as well as organ transplant tolerance. This is a preview of subscription content, access via your institution Prices may be subject to local taxes which are calculated during checkout Liuhui Fu, Rabi Upadhyay, Maria Pokrovskii, Francis M. Chen, Gabriela Romero-Meza & Dan R. Littman You can also search for this author inPubMed Google Scholar Fu, L., Upadhyay, R., Pokrovskii, M. et al. Prdm16-dependent antigen-presenting cells induce tolerance to gut antigens. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

You are using a browser version with limited support for CSS. João Vieira is a postdoc at the University of Cologne, Germany. You can also search for this author in PubMed Google Scholar You have full access to this article via your institution. People protesting against Brazilian president Jair Bolsonaro's education-budget cuts, in São Paulo on 13 August 2019.Credit: Cris Faga/NurPhoto/Getty My laboratory, based in the coastal region of Macaé, became collateral damage in a war on science declared by Jair Bolsonaro, our president from 2019 to 2022 — a war that might feel familiar to many US researchers now that their own president seems determined to cut funding for scientific research. After Bolsonaro came to power in 2019, more than 5,600 grants vanished under sweeping austerity measures that targeted research and higher education. The cuts left thousands of researchers in limbo as national funding agencies such as the Brazilian Federal Agency for Support and Evaluation of Graduate Education and Brazilian National Council for Scientific and Technological Development slashed their budgets. Faculty members scrambled for emergency support while researchers rationed reagents and faced the prospect of stalled projects. Without warning, she found herself locked out of the lab. Desperate not to lose everything she had worked for, she spoke out, and the story of her cancelled funding struck a nerve with the public. Support came together quickly enough to keep her from walking away, but only just. Another, after months of uncertainty, moved abroad to continue his research. The cuts sparked nationwide protests led by students, professors and scientific societies, with banners in São Paulo, Rio de Janeiro and Brasília declaring: “Knowledge is not an expense.” Marching with thousands — even in my small city — I felt a powerful unity. On 18 October, 2019, the Brazilian Ministry of Education unlocked one billion Brazilian reais (about US$172 million) in funds for federal universities — a rare concession that groups such as the Brazil National Union of Students credited to our mobilization. I was part of a movement that had pushed back against the cuts and reaffirmed the role of education and research in Brazil. By June, as Bolsonaro's administration continued to dismiss science as an elitist pursuit, our lab shifted from evolutionary research to processing COVID-19 tests with Macaé's city hall. Our qPCR machines, once mapping beetle gene expression, were repurposed for diagnoses in a testing effort that helped to keep Macaé's fatality rate below the state average. This crucial initiative meant that I lost access to the institute — not only because it became a diagnostic lab but also owing to a strict sanitary barrier. In July that year, I secured funding for an eight-month internship at evolutionary biologist Siegfried Roth's lab at the University of Cologne, Germany — an important opportunity that finally provided me with the resources to complete my PhD. By then, I had booked flights, rented an apartment and spent nights reading catastrophic headlines detailing Bolsonaro's relentless attacks on science — dismissing the virus as “just a little flu”, promoting hydroxychloroquine as a treatment despite overwhelming evidence of its ineffectiveness and systematically slashing science budgets and environmental protections across the Amazon rainforest. Stuck in limbo, I again adapted by shifting my research focus from wet lab work to bioinformatics, analysing data sets from home and collaborating through Zoom with Siegfried's group while waiting for borders to reopen. I finally arrived in late September 2021, and my official internship ran from October that year to April 2022. Meanwhile, online trolls, and even some family members, mocked my research as frivolous, championed funding cuts and embraced debunked so-called COVID-19 cures such as hydroxychloroquine. Amid the chaos, I learnt to thrive in uncertainty — skills that no grant could fund. Those years blurred together in setbacks and personal challenges, each day testing my resolve amid constant political turmoil. My advisers, Rodrigo Nunes da Fonseca and Helena Araújo, despite facing their own institutional chaos, found time to strategize about grants and training opportunities. He claimed that the election was rigged, and on 8 January, a mob of his supporters stormed government buildings in Brasília. The author received funding from CAPES (Coordination for the Improvement of Higher EducationPersonnel) and FAPERJ (Carlos Chagas Filho Foundation for Research Support of the State ofRio de Janeiro) during their PhD to support research in evolutionary developmental genetics.The author currently does not receive funding from these agencies. ‘All this is in crisis': US universities curtail staff and spending as Trump cuts take hold Trump team ‘survey' sent to overseas researchers prompts foreign-interference fears NIH cuts triggered a host of lawsuits: Nature's guide to what's next A mental-health crisis plagues PhDs — these evidence-led initiatives offer help Norway set to scrap mandatory language training for foreign postdocs and PhD students Work time part-time Start date 01.06.2025 Employment period limited Application deadline 30.04.2025 Deployment location Charité Campus Charit... The School of Engineering (SOE) at Westlake University is seeking to fill multiple tenured or tenure-track faculty positions in all ranks. ‘All this is in crisis': US universities curtail staff and spending as Trump cuts take hold Trump team ‘survey' sent to overseas researchers prompts foreign-interference fears An essential round-up of science news, opinion and analysis, delivered to your inbox every weekday. Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

This tiny, elusive particle with no electric charge likely permeates every corner of the universe, but you'd be hard-pressed to know that without extremely specialized instruments. Trillions pass through you every second, in fact, all without interacting with a single atom of your body. That is but one of the reasons why, for something so supposedly abundant and fundamental, we know painfully little about the neutrino—not even something so basic as its mass. These results, published last week in Science, represent just a fraction of KATRIN's investigations; about three quarters of the detector's planned data haul from its ongoing 1,000-day campaign remains to be analyzed and revealed. Another reason for excitement is that KATRIN has achieved a twofold increase in sensitivity from just last year, when some researchers raised questions as to whether the experiment would even be able to make progress on physicists' decades-long quest to gauge the neutrino's mass. By the conclusion of KATRIN's campaign, he says, the detector's sensitivity is targeted for a lower-end neutrino mass of 0.3 eV, another significant boost. If you're enjoying this article, consider supporting our award-winning journalism by subscribing. By purchasing a subscription you are helping to ensure the future of impactful stories about the discoveries and ideas shaping our world today. With their eyes on that prize, for this particular round of data analysis, Lokhov and his colleagues, including co-author Christoph Wiesinger, performed several technical overhauls to significantly improve the instrument's capabilities. “Now KATRIN is in a more stable, near-final configuration, [so] I'm very confident we'll manage this [sensitivity boost] in upcoming years.” It seeks to constrain the neutrino's mass by looking at the energy spectrum of electrons and electron antineutrinos emitted by decaying tritium, a radioactive isotope of hydrogen. “The trick is that to produce a neutrino in this decay process, one needs to at least produce a mass that a small, nonzero mass [that] would influence how much energy [would be] left for the electrons.” And that “leftover” energy, he says, would hint at the presence of something else—the neutrino—present in the decay process. Famously, almost all of this theory's predictions have been experimentally confirmed, yet some of its forecasts for the neutrino have notoriously fallen flat. The model predicts that neutrinos should be completely massless, but this was ultimately refuted by a Nobel-winning experiment that showed neutrinos not only have mass but also, for whatever reason, change mass by oscillating between three different neutrino varieties, or “flavors.” “You know, when everything is settled and we are all happy, [neutrinos] are like that one person in the room saying, no, not quite,” muses Carlos Argüelles Delgado, a physicist at Harvard University, who is unaffiliated with KATRIN. But the neutrino's troublesome nature is precisely why physicists are so enamored with it; the tiny particle, theorists say, must be a rebel with a cause, with some deeper and more fundamental explanation for its quirks that could open vast new realms of physics beyond the Standard Model's increasingly bland confines. And results like KATRIN's are part of a steady flow of theoretical and experimental advances bringing us incrementally closer to those long-awaited breakthroughs. “There's a tricky business here because the neutrinos are superpositions of mass states—they have three—and what [KATRIN] shows is that this mass combination can be no larger than 0.45 eV,” Argüelles Delgado says. That said, KATRIN is probably going to be the last of its kind, Argüelles Delgado says, noting the diminishing returns associated with scaling up such experiments. Major investments to make bigger, longer-running experiments risk only delivering marginal advances—which is all the more reason why KATRIN's sunk costs and ongoing success now call for urgency. If or when KATRIN achieves its intended higher sensitivity, this particular experiment will end—but the hope is that future instruments will be able to continue its mission by taking heed of what physicists have learned from KATRIN. Despite its end, the data will be a treasure trove that physicists will mine for discoveries for many years to come. “But even though that the [neutrino's] mass is so small, we expect today that just by there being so many [of them], they have a large influence on the cosmos—how structures are forming and how they evolve.” It's fascinating to realize that such an infinitesimal and rebellious particle can have such profound effects on both subatomic and cosmic scales—and that it can be robustly studied in earthbound laboratories at all. “Neutrinos are one-of-a-kind portals to new discoveries in physics—they were always like this from the beginning, when they were first postulated,” Lokhov says. “And even now, they're still bringing some new, exciting discoveries that [further] our understanding of nature.” Gayoung Lee is Scientific American's current news intern. A philosopher turned journalist, originally from South Korea, Lee's interests lie in finding unexpected connections between life and science, particularly in theoretical physics and mathematics.

It's essentially a “major category” of living things that groups organisms together based on shared qualities. “This zone supports most life on the planet as it regulates essential processes like soil formation, water cycling, and nutrient cycling, which are vital for food production, water quality, and ecosystem health,” James Tiedje—researcher on the study—said in a press release. “Despite its importance, the deep Critical Zone is a new frontier because it's a major part of the Earth that is relatively unexplored.” Researchers identified CSP1-3 in soil samples from China and Iowa. Yes, the two locations seem unrelated—but that was intentional. Researchers suggest that the organisms underwent at least one transition to colonize soil environments throughout its evolutionary history. ​​ “Most people would think that these organisms are just like spores or dormant,” Tiedje said in the release. “But one of our key findings we found through examining their DNA is that these microbes are active and slowly growing.” Researchers were also surprised by the sheer amount of these microbes which, in some cases, made up over 50% of the soil's population. This level of dominance is not present in topsoil, and Tiedje explains that this is likely because the bacteria has evolved to thrive in the destitute deep soil conditions over many centuries. This research could prove important for ongoing studies attempting to solve some of the issues we are facing today. “CSP1-3's physiology, driven by their biochemistry, is different, so there may be some interesting genes of value for other purposes,” Tiedje said. Her work appears in several publications including Biography.com and Popular Mechanics. When she's not writing, Emma can be found hopping between coffee shops on the hunt for the world's best oat milk cappuccino. This Ancient Larva Still Has Its Brain And Guts An Enormous Desert Used to Be a 138-Foot-Deep Lake This “Battery In a Rock” Changes the Energy Game This Dark Ocean Pit Has a Bleak Weather Forecast A DNA Mutation Helps Some Fish Survive Deep Waters This Bizarre Fossil Is a Whole New Form of Life

Replacing Federal Workers with Chatbots Would Be a Dystopian Nightmare The Trump administration sees an AI-driven federal workforce as more efficient. Instead, with chatbots unable to carry out critical tasks, it would be a diabolical mess Imagine calling the Social Security Administration and asking, “Where is my April payment?” only to have a chatbot respond, “Canceling all future payments.” Your check has just fallen victim to “hallucination,” a phenomenon in which an automatic speech recognition system outputs text that bears little or no relation to the input. Hallucinations are one of the many issues that plague so-called generative artificial intelligence systems like OpenAI's ChatGPT, xAI's Grok, Anthropic's Claude or Meta's Llama. If these automated systems can't even reliably parrot back the exact information that is given to them, then their outputs will be riddled with errors, leading to inappropriate and even dangerous actions. Automated systems cannot be trusted to make decisions the way that federal workers—actual people—can. If you're enjoying this article, consider supporting our award-winning journalism by subscribing. Historically, “hallucination” hasn't been a major issue in speech recognition. That is, although earlier systems could take specific phrases and respond with transcription errors in specific phrases or misspell words, they didn't produce large chunks of fluent and grammatically correct texts that weren't uttered in the corresponding audio inputs. But researchers have shown that recent speech recognition systems like OpenAI's Whisper can produce entirely fabricated transcriptions. Whisper is a model that has been integrated into some versions of ChatGPT, OpenAI's famous chatbot. For example, researchers from four universities analyzed short snippets of audio transcribed by Whisper, and found completely fabricated sentences, with some transcripts inventing the races of the people being spoken about, and others even attributing murder to them. I'm sure he didn't have a terror knife so he killed a number of people.” In another example, “two other girls and one lady” was transcribed as “two other girls and one lady, um, which were Black.” In the age of unbridled AI hype, with the likes of Elon Musk claiming to build a “maximally truth-seeking AI,” how did we come to have less reliable speech recognition systems than we did before? The answer is that while researchers working to improve speech recognition systems used their contextual knowledge to create models uniquely appropriate for performing that specific task, companies like OpenAI and xAI are claiming that they are building something akin to “one model for everything” that can perform many tasks, including, according to OpenAI, “tackling complex problems in science, coding, math, and similar fields.” To do this, these companies use model architectures that they believe can be used for many different tasks and train these models on vast amounts of noisy, uncurated data, instead of using system architectures and training and evaluation datasets that best fit a specific task at hand. A tool that supposedly does everything won't be able to do it well. Whisper simultaneously maps the input speech to text and predicts what immediately comes next, a “token” as output. So giving the system two disparate jobs to do, speech transcription and next-token prediction, in conjunction with the large messy datasets used to train it, makes it more likely that hallucinations will happen. Like many of OpenAI's projects, Whisper's development was influenced by an outlook that its former chief scientist has summarized as “If you have a big dataset and you train a very big neural network,” it will work better. Given that its decoder is tasked with both transcription and token prediction, without precise alignment between audio and text during training, the model can prioritize generating fluent text over accurately transcribing the input. And unlike misspellings or other mistakes, large swaths of coherent text don't give the reader clues that the transcriptions could be inaccurate, potentially leading users to use them in high-stakes scenarios without ever finding their failures. Conversely, OpenAI's attempt to use a “general” model architecture rather than one tailored for speech transcription—sidestepping the time and resources it takes to curate data and adequately compensate data workers and creators—results in a dangerously unreliable speech recognition system. If the current one-model-for-everything paradigm has failed in the context of English language speech transcription that most English speakers can perfectly perform without further education, how will we fare if the U.S. DOGE Service succeeds in replacing expert federal workers with generative AI systems? We cannot afford to replace the critical tasks of federal workers with models that completely make stuff up. There is no substitute for the expertise of federal workers handling sensitive information and working on life-critical sectors ranging from health care to immigration. His work at Lesan focuses on building advanced speech recognition and machine translation systems for underrepresented languages.

Physicist Richard Lieu first explored the idea that gravity could exist without mass—now he's got a new cosmological model that eschews the need for dark energy. But there was a problem: our current understanding of physics couldn't explain this phenomenon. Scientists hypothesized that there must be some unknown “dark energy” in the universe that propels the universe ever outward, but as theoretical cosmologist Katie Mack states: “We can't see it, we don't know what it is, and we're not even sure how it can exist at all.” In the decades following this discovery, independent pieces of evidence have supported the idea, but this elusive form of energy—estimated to make up 70 percent of the universe—has still never been directly observed or measured. Because science abhors an epistemological vacuum, alternative theories of this universal acceleration have emerged in an attempt to circumvent this dark energy conundrum with names like Modified Newtonian Dynamics (or MOND) and “timescape cosmology,” the latter of which essentially attributes dark energy to a misunderstanding of kinetic energy expansion at universal scales. Lieu isn't a stranger to out-of-the-box cosmological thinking, grabbing headlines just last year for intimating that gravity can exist without mass (though, the idea relied on negative mass, which is as hypothetical as dark energy). This new study builds a cosmological model around the central idea being that universal acceleration is caused by multiple singularities blinking in and out of existence. “The new model can account for both structure formation and stability, and the key observational properties of the expansion of the universe at large, by enlisting density singularities in time that uniformly affect all space to replace conventional dark matter and dark energy,” Lieu said in a press statement. These “transient temporal singularities,” as Lieu calls them, create step-like bursts that periodically flood the universe with matter and energy. Like every other alternative theory that forgoes dark energy, this model has no empirical data to back up its claims. Thankfully, the Nancy Grace Roman Space Telescope (which launches in 2027) is purpose-built to explore the cosmos for evidence of dark energy. But it will require cold, hard data to prove whether dark energy—or some other alternative, like multiple singularities—is the long-sought-after energy force behind the unexplainable. Experts Think They've Seen Hints of String Theory Everything We Knew About the Cosmos Might Be Wrong Scientists Now Know Why This Black Hole is Pulsing One Type of Dark Matter Could Break the Universe

A fluid study homes in on the best method to make a cup of coffee, scientists use the Hubble Telescope to reassess the length of a day on Uranus, and we discuss more of the latest in science in this news roundup. For Scientific American's Science Quickly, I'm Rachel Feltman. Let's catch up on some of the science news you might have missed last week. But a study published last week in the journal Physics of Fluids brings some actual science into debates over how to brew the perfect pot of joe. Using transparent silica gel particles in place of coffee grounds, researchers captured high-speed footage showing exactly how water flows through a pour-over setup under different conditions. They determined that the best way to brew a strong cup of coffee was to maximize the contact time between water and coffee grounds while also allowing for plenty of mixing so as much coffee as possible was extracted. As those dudes from parties in Brooklyn have probably already told you. If you get it right, the researchers say, you can actually get a stronger cup of coffee using a smaller quantity of grounds. If you're enjoying this article, consider supporting our award-winning journalism by subscribing. By purchasing a subscription you are helping to ensure the future of impactful stories about the discoveries and ideas shaping our world today. Now that we're all bright-eyed and bushy-tailed, let's move on to another troubling story of cuts in federal funding for research. Last Tuesday the U.S. Department of Commerce announced that the Trump administration will pull around $4 million in research grants for climate change-related projects from Princeton University. One of the targeted projects focuses on how water supplies might fluctuate as global warming progresses. The Department of Commerce stated that “using federal funds to perpetuate these narratives does not align with the priorities of this Administration,” which is, frankly, chilling language to use when talking about climate change research. The press release also accused some of the slashed projects of increasing “climate anxiety,” which is a phrase that's increasingly being used to cast folks' concerns over very real evidence about the climate crisis in a hysterical light. Speaking of environmental threats: a study published last Thursday in the journal Communications Earth & Environment found that less than 10 percent of the plastic made worldwide in 2022 contained recycled materials. The world produced more than 400 million tons of plastic that year. And some estimates say that amount will more than double by 2050. The new study also found that just around 28 percent of all plastic waste made it to the sorting stage and only half of that plastic was actually recycled. On average, each individual in the U.S. consumed about 476 pounds [216 kilograms] of plastic that year. Now, obviously plastic usage is a massive, complex, systemic problem that high income countries around the world need to address, so this isn't me trying to make you feel guilty about your ever-growing pile of old takeout containers. The Small Magellanic Cloud is a galaxy not far from our own, and a new study published in the Astrophysical Journal Supplement Series suggests that things might be getting a little hectic over there. In other space news, it turns out that a day on Uranus lasts slightly longer than we thought. A study published last Monday in Nature Astronomyused data from the Hubble Space Telescope to estimate the ice giant's rotation rate with unprecedented accuracy. That figure relied on measurements of the planet's magnetic field and radio signals emitted by its auroras. For a better estimate scientists used more than a decade's worth of Hubble data to track the movement of Uranus's auroras, which helped them zero in on the actual location of the planet's magnetic poles. The researchers' findings added a whopping 28 seconds to Uranus's previously estimated rotational period. Some earlier research has suggested that dinosaurs were already on the outs before that infamous asteroid struck the killing blow. But a study published last Tuesday in Current Biologyargues that the dinosaurs were doing just fine before that pesky space rock came along, thank you very much. That fossil record does indeed seem to show that dinosaur populations started declining millions of years before the asteroid hit. The researchers argue that geological changes made dinosaur fossils less likely to be preserved in places where archaeologists could one day access them. It's certainly not the end of this debate, but it's now a little more plausible to imagine that, had things gone down a little differently, we might still have dinosaurs roaming the Earth today—other than birds, of course. We'll be back on Wednesday to talk about a trendy disinfectant that sounds almost too good to be true: hypochlorous acid. Tune in to get the full scoop on this so-called miracle molecule. Science Quickly is produced by me, Rachel Feltman, along with Fonda Mwangi, Kelso Harper, Naeem Amarsy and Jeff DelViscio. Shayna Posses and Aaron Shattuck fact-check our show. Our theme music was composed by Dominic Smith. Subscribe to Scientific American for more up-to-date and in-depth science news. Rachel Feltman is former executive editor of Popular Science and forever host of the podcast The Weirdest Thing I Learned This Week. Fonda Mwangi is a multimedia editor at Scientific American. She previously worked as an audio producer at Axios, The Recount and WTOP News. He has worked on projects for Bloomberg, Axios, Crooked Media and Spotify, among others.

You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. While it has been suggested that alterations in the composition of gut microbial metabolites may play a causative role in the pathophysiology of autism spectrum disorder (ASD), it is not known how gut microbial metabolites are associated with ASD-specific brain alterations. In this cross-sectional, case-control observational study, (i) fecal metabolomics, (ii) task-based functional magnetic resonance imaging (fMRI), and (iii) behavioral assessments were obtained from 43 ASD and 41 neurotypical (NT) children, aged 8–17. The fMRI tasks used socio-emotional and sensory paradigms that commonly reveal strong evoked brain differences in ASD participants. Our results show that fecal levels of specific tryptophan-related metabolites, including kynurenate, were significantly lower in ASD compared to NT, and were associated with: 1) alterations in insular and cingulate cortical activity previously implicated in ASD; and 2) ASD severity and symptoms (e.g., ADOS scores, disgust propensity, and sensory sensitivities). Thus, we identify associations between gut microbial tryptophan metabolites, ASD symptoms, and brain activity in humans, particularly in brain regions associated with interoceptive processing. The gut microbial ecosystem generates an estimated 40% of all metabolites in the body's circulation, including neuroactive and inflammatory molecules1,2. Within the brain-gut-microbiome (BGM) system, neuroactive gut metabolites can modulate brain activity directly, via the systemic circulation, or via vagal and spinal afferents. Ninety percent of vagal fibers are afferents, underscoring the magnitude of information that is transmitted from the gut, its microbiome, and from vagal signals to the brain3,4. Together, these regulatory signals from the gut microbiome can influence socio-emotional and sensory processing, cognition, and behavior5,6,7,8. Pre- and postnatal microbial and neural disruptions may have profound effects on the development of the enteric and central nervous system9. Such disruptions have been linked to the etiology of neurodevelopmental disorders, such as autism spectrum disorder (ASD)10,11,12,13,14. Indeed, longitudinal studies in ASD indicate that gut microbial dysbiosis can be found in infancy15 and persist through adulthood16,17. Early life gut dysbiosis may be associated with compromised barriers within the BGM system, contributing to the development of neurodevelopmental disorders13,18,19. Further, there is evidence from cross-sectional human studies indicating that gut microbiome and metabolite alterations correlate with ASD symptoms (i.e., difficulties in socio-emotional behavior and sensory perception), which can be ameliorated with probiotics or fecal microbiota transplants from neurotypical (NT) children20,21,22,23. The high prevalence of gastrointestinal (GI) symptoms in ASD (46–84%) further underscores the gut as an important component in ASD pathophysiology3,5,24,25. Of the gut microbial metabolites that may be relevant to ASD pathophysiology, there has long been an interest in those in the tryptophan/serotonin pathway (Fig. Roughly 30% of individuals with ASD show elevated blood serotonin levels, which have been linked to dysregulation of symptoms such as mood, appetite, and social interactions26. In addition, individuals with ASD with hyper-serotonemia are more likely to experience GI issues27,28. However, reducing dietary tryptophan, which is the precursor for 95% of the body's serotonin, has been associated with increased ASD symptomatology29,30,31,32. Serotonin, generated by intestinal enterochromaffin cells, is almost completely taken up by platelets. However, as serotonin does not cross the blood–brain barrier, serotonin signals are thought to reach the brain via serotonin-mediated vagal signaling26,33,34. Gut microbes also play an important role in the metabolism of dietary tryptophan into indoles, kynurenate (KA) and kynurenine (KYN), which, in contrast to serotonin, are able to cross the blood–brain-barrier. While KA has neuroprotective effects, KYN has neurotoxic effects especially during critical periods of development, influencing brain microstructure, activity, and development5,35,36. Thus, there is evidence that dysregulation in the tryptophan metabolism and KYN pathway may influence ASD symptomatology both by peripheral and central mechanisms, at least in a subset of individuals. Some animal studies have examined how the brain may mediate relationships between gut metabolites and behavior13,37. However, to date, there are no human ASD studies that have looked at interactions between gut metabolites and brain activity, and their impact on ASD symptomatology. In this cross-sectional comparative study, 43 ASD and 41 neurotypical (NT) youth (aged 8–17) underwent functional magnetic resonance imaging studies (fMRI), fecal metabolomics focused on the tryptophan pathway, and comprehensive clinical and behavioral assessments. We focused on interoceptive, emotional, and sensory processing brain regions of interest (ROIs) based on prior studies in children with ASD and their relevance to vagal processing (see “Methods: fMRI Tasks”). The fMRI tasks all involved socio-emotional processing (processing emotions, facial expressions, others' somatosensory experiences); such socio-emotional processing has been shown to be modulated by tryptophan metabolites, like serotonin31,32,38. Our primary hypotheses were that the relative abundances of tryptophan-related gut metabolites differs between ASD and NT groups, and correlate with ASD symptomatology as well as with atypical brain activity in regions consistently implicated in ASD (i.e., insular subregions, pregenual anterior, and mid-cingulate). While the study design did not allow us to address causality, we additionally explored the hypothesis that task-based brain activity mediates metabolite and behavioral relationships. Independent samples t-tests and Fisher's exact tests were used to determine significant ASD-NT differences in demographic and behavioral variables. As shown in Supplemental Table 1, compared to the NT group (N = 41; 20F; mean age = 11.6), the ASD group (N = 43; 11F; mean age = 12.06) showed significantly higher values for: body mass index (BMI), prenatal antibiotic use, gastrointestinal (GI) symptoms, sensory sensitivities, attention deficit/hyperactivity disorder (ADHD) symptoms, disgust sensitivity, disgust propensity, and social difficulties. In addition, the ASD group showed significantly lower scores on the full-scale intelligence quotient (FSIQ) and sleep quality (all ps < 0.05). No between-group differences in diet were found (see Supplemental Table 1 and Supplemental fig. Refer to Supplemental Table 2 for the participant's race and ethnicity by group. Regarding stool-derived metabolites, as compared to the NT group, the ASD group displayed significantly lower KA levels (β = 0.78, se = 0.23, q = 0.02, Cohen's d = 0.82; Fig. Other tryptophan pathway metabolites such as xanthurenate (Cohen's d = 0.49), 5-hydroxypicolinic acid (Cohen's d = 0.41), and indolepropionate (Cohen's d = 0.39) had small to moderate effect sizes but did not achieve FDR-corrected statistical significance at q < 0.05 (Supplemental Table 3). A contrast analysis within the framework of the GLM indicated significant differences between ASD (N = 43) and NT (N = 41) groups in kynurenate (KA; q = 0.02, two-tailed). Given that in our sample there were greater reports of GI symptoms in ASD compared to NT, in a subset of participants with this data available, we performed a sensitivity analysis including GI symptoms as a covariate. The significant group differences in KA abundance persisted (β = 1.11, se = 0.29, q = 0.009, Cohen's d = 1.15). Further, indoleacrylate (Cohen's d = 0.76), indolepropionate (Cohen's d = 0.62), 5-hydroxypicolinic acid (Cohen's d = 0.58), and xanthurenate (Cohen's d = 0.53) had large effect sizes but did not achieve FDR-corrected statistical significance at q < 0.05 (Supplemental Table 4). In addition, including diet as a covariate did not change the results. We primarily focused on brain regions involved in interoceptive, vagal, and emotion processing, as these may be especially impacted in altered gut-brain interactions. In addition, brain regions of interest (ROIs) were selected from those which showed significant differences in ASD compared to NT based on prior studies and our fMRI tasks (tasks included: processing others' facial expressions/actions; disgust processing; processing somatosensory experiences; see Methods: fMRI Tasks and Supplemental Table 5). 3 shows, ROIs included the: cingulate cortex (right mid-cingulate, pregenual anterior cingulate, anterior mid-cingulate cortex/dorsal medial prefrontal cortex), bilateral insula (anterior, dorsal anterior, mid, ventral, posterior), right fusiform face area (FFA), right inferior gyrus pars opercularis (IFGop), and right primary somatosensory cortex (S1). While our predominant focus was on subregions of the insula and cingulate due to their involvement in interoceptive and emotional processing, we additionally considered other ROIs with significant ASD vs. NT differences in our fMRI tasks and from prior ASD studies. Please see “Methods” for how ROIs were selected. R Right, L Left, insular subregions (dAI left dorsal anterior insula, vAI ventral anterior insula, MI mid-insula, PI posterior insula); cingulate subregions (pACC pregenual anterior cingulate cortex, MCC mid-cingulate cortex, dmPFC/aMCC dorsal medial prefrontal cortex/anterior mid-cingulate cortex); IFGop inferior frontal gyrus, pars opercularis, S1 primary somatosensory cortex. General linear models (GLMs) were applied to assess associations between brain regions showing differences on the stimulus-evoked tasks with metabolites, controlling for age, sex, FSIQ, and BMI. During facial expression processing, increased activity in the right IFGop was significantly associated with higher levels of anthranilate, while decreased activity in the right mid-cingulate cortex (MCC) was significantly associated with higher levels of tryptophan betaine. Activity in the right MCC when processing hand actions was significantly associated with increased abundance of N-acetyltryptophan. In addition, higher right MCC activity during processing of non-emotional faces was significantly associated with increased c-glycosyltryptophan. For the disgust processing task, significant ROI-metabolite correlations primarily involved insular subregions. Notably, lower levels of indolelactate were significantly associated with increased activity in the right mid-insula when viewing disgusting foods. When viewing disgust facial expressions, increased activity in the left dorsal anterior insula was associated with higher levels of KA. For the somatosensory task, increased levels of indole-3-carboxylate were significantly associated with increased activity in right S1 and dorsal medial prefrontal cortex /anterior mid-cingulate cortex (dmPFC/aMCC) when processing object touch. By contrast, decreased levels of indole-3-carboxylate were associated with activity in the left posterior insula when processing social touch. Brain-behavioral association results within the ASD group are shown in Table 2 (see Supplemental Table 6 for additional significant associations and Supplemental Table 10 for NT results). Of note, increased right MCC activity related to non-emotional facial processing was positively associated with disgust sensitivity across various stimuli conditions. Further, when looking at disgusting foods, decreased activity in the right mid-insula was significantly associated with autism severity (ADOS-2 RRB and ADOS-2 total score), and activity in the left ventral anterior insula was associated with restricted, repetitive, and stereotyped patterns of behavior (ADI-R RRB). The above results indicated that 7 tryptophan metabolites were significantly correlated with alterations in brain activity in ASD. We applied GLMs to investigate how these 7 metabolites may also be related to behavior in the ASD group, while controlling for age, sex, BMI, and FSIQ. As Table 3 shows, we found large effect size associations between metabolites and behavioral variables. Higher levels of tryptophol were significantly correlated with sensory sensitivities (vestibular/proprioception, social, and non-social). In addition, higher levels of anthranilate were significantly correlated with greater quality of sleep. Refer to Supplementary Table 7 for the results of a post hoc analysis of tryptophan metabolite-behavior associations within ASD with all tryptophan metabolites. NT results are presented in Supplemental Table 9. Based on previous theoretical work and animal studies indicating that metabolites affect brain activity which in turn affect behavior13,37,39, we conducted exploratory mediation analyses, with the brain activity in regions that differed between ASD and NT as the mediator between tryptophan metabolite-behavior relationships. 4 shows, mediation models (controlling for brain-related variables, age and FSIQ) indicated significant mediating effects for the right mid-insula and the right mid-cingulate. Specifically, activity in the right mid-insula during disgust processing statistically mediated the relationship between indolelactate and ASD severity as measured by the ADOS RRB score (indirect effect: Std. β = 0.341, SE = 0.227, 95% CI [0.016,0.967] and ADOS total score (indirect effect: Std.β = 0.440, SE = 0.585, 95% CI [0.005, 0.984]). In addition, activity in the right mid-cingulate during facial expression processing mediated the relationship between tryptophan betaine and disgust sensitivity (indirect effect: Std. We note that while the significant statistical mediation effects observed in these models are consistent with the conceptual model whereby gut metabolites impact brain activity increasing ASD symptomatology, longitudinal and/or interventional experiments (e.g., fecal transplant studies at critical periods of development) are necessary to determine causation. A summary of the main results in ASD is displayed in Table 4. The summary of NT results is available in Supplemental Table 11. For each mediation model, the figure contains the standardized beta and standard error: Std. β (SE) for direct effects. A Right mid-insula: disgusting foods vs. rest, indolelactate, and ADOS RRB. B Right mid-insula: disgusting foods vs. rest, indolelactate, and ADOS total score. C right mid-cingulate: non-emotional faces vs. rest, tryptophan betaine, and disgust sensitivity. ADOS Autism Diagnosis Observation Schedule, ADI-R Autism Diagnostic Interview-Revised, RRB Restricted and Repetitive Behaviors, DPSS-R Disgust Propensity and Sensitivity Scale - Revised, r_MI right mid-insula, MCC mid-cingulate cortex. The current results confirmed our main hypotheses that tryptophan-related fecal metabolites differ between ASD and NT groups and show significant associations with known ASD brain alterations as well as symptomatology, with medium to large effect sizes. Here, we report data consistent with the conceptual model that brain activity mediates the relationship between tryptophan metabolites and ASD symptomatology. Although the current study was not designed to demonstrate causality, these findings represent an important step toward a better mechanistic understanding of ASD. 1 shows, in the gut, more than 90% of dietary tryptophan is metabolized into kynurenine (KYN) and to a lesser degree into serotonin as well as other metabolites. Importantly, any changes in regulation of the KYN pathway, in particular a reduction in plasma levels of kynurenate (KA) and an increase in the KYN/KA ratio, can lead to neurotoxic effects, impact blood serotonin levels, and have profound behavioral and cognitive effects40. Indeed, such dysregulation has been associated with several neuropsychiatric and neurological disorders such as ASD, schizophrenia, depression, and epilepsy41,42,43,44. Here, we found significantly decreased levels of KA in our ASD group compared to NT. This result is consistent with prior research by Bryn et al. (2018) showing lower blood levels of KA in ASD compared to NT children. There are conflicting data about whether differences in plasma and/or fecal metabolites in ASD may be driven by the high degree of ASD participants with GI issues23,45. However, consistent with Needham et al. (2021), we did not find any significant relationships between fecal KA levels and GI distress in ASD, indicating that our results may not be limited to those with GI symptoms. Whether KA abundance can be used as a biomarker of ASD remains a question for future research23. In this study, we focused on interactions of tryptophan metabolites with brain activity specifically in brain regions important for interoceptive, vagal, disgust, and socio-emotional processing which have previously been implicated in ASD38,46,47,48,49. Indeed, activity in these ROIs was significantly related to ASD symptomatology and severity (see Table 2). Importantly, we show that in ASD, activity in these ROIs is significantly correlated with fecal abundance of gut-derived metabolites in the tryptophan pathway, including indoles, which have been implicated in previous ASD research50,51. Specifically, we found that different fecal tryptophan metabolites are significantly correlated with task-based brain activity (left pregenual anterior and middle cingulate cortex, insular subregions, right S1) and with ASD severity and symptomatology. Notably, in line with our hypothesis, we found that in ASD, brain activity in the mid-cingulate associated with social processing modulated the relationship between tryptophan betaine and disgust sensitivity. Tryptophan-related metabolites (i.e., indoles, serotonin) are important peripheral modulators of vagal activity52. Their altered abundance in the gut has been significantly linked to a number of psychological, neurological, and medical symptoms, such as memory loss, long COVID, depression, sleep disturbances, and anxiety52,53. Thus, the altered abundance of fecal tryptophan-related metabolites may modify activity in vagal and enteric afferent terminals in the gut, or after absorption, directly activate brainstem nuclei, influencing upstream MCC brain activity during socio-emotional tasks and impacting emotional processing. The current data support a hypothesized mechanistic link between common ASD differences in brain activity (MCC, insula) and behavior (disgust sensitivity)49,54. Further, in the ASD group, we specifically found several correlations between indole metabolites, brain activity (insular subregions, dmPFC/aMCC, IFGop, and S1), and symptomatology (autism severity [ADI-R RSI] and alexithymia). In contrast to serotonin, indoles are exclusively generated by gut microbes from tryptophan and therefore strongly implicate gut microbial alterations in ASD symptomatology55. Interestingly, supporting our last hypothesis, we found that disgust processing activity in the right mid-insula mediates the relationship between indolelactate and two measures of autism severity (ADOS total score and ADOS RRBs). This is particularly noteworthy given that: 1) disgust processing is strongly related to interoceptive processing, making it particularly suited for gut metabolite influence; 2) children with ASD commonly have differences in disgust processing49,54; and 3) the mid-insula is a hub of interoceptive, emotion, and chemosensory processing, and is particularly known to show atypical activity and connectivity in ASD46,54,56. Thus, although these findings do not prove causality between gut microbial metabolites and brain and behavior alterations, they are consistent with the concept that indole metabolites generated by gut microbes (and other tryptophan-related metabolites) directly or indirectly impact brain function, contributing to some ASD-related behavioral symptomatology. Prior studies have also found that differences in levels of fecal indole metabolites in ASD correlate with autism symptomatology23,57,58. For example, Needham et al. (2021) found significant negative correlations between fecal levels of several indoles, such as indolepropionate, indole, n-formlyanthranalic acid, and indole-3-carboxylate and ASD severity (ADI-R)23. The current results expand upon these previous findings by adding that activity in the right mid-insula may be an important mediator between gut microbial generated indole metabolite and ASD behavior. Interestingly, we found that a history of prenatal antibiotic exposure was significantly higher in the ASD group. Prenatal maternal antibiotic exposure has been shown to affect the maternal microbiome, which plays an important role in the fetal brain's exposure to altered maternal microbial metabolites. This prenatal mechanism has been hypothesized to play a role in the etiology of ASD14,59. However, in a post hoc analysis using independent samples t-tests, we found no significant differences in metabolite abundances in the tryptophan pathway between ASD participants with and without prenatal antibiotic exposure; further studies are needed on this topic. There are several important limitations to the current study. We had strict inclusion criteria for the fMRI component (right handedness, FSIQ > 79, 8–17-year-olds, exclusion of other neuropsychiatric and neurological disorders), which limited the sample heterogeneity and size. To account for this, we utilized a hypothesis-driven approach considering only metabolites within the tryptophan pathway and a priori brain ROIs to limit the number of comparisons, as well as multiple comparisons corrections in all GLM analyses. Another limitation is that we had a larger ratio of males to females in our ASD group compared to the NT group. This was largely due to the exceptional challenge of enrolling in-person participants during the COVID-19 pandemic, which made our initial effort to match groups by sex unviable in a timely manner. However, we accounted for this by including sex as a covariate in all our analyses. Further, the study design was cross-sectional and did not allow the assessment of a causal relationship of the gut microbial metabolites with the brain or behavior. While the mediation models are consistent with our hypotheses, they do not prove causality. Future replication studies are needed as well as studies with more heterogeneous, larger samples with groups matched in sex, well-powered longitudinal designs aimed at studying critical periods of development, interventional designs to demonstrate causality, analysis of broader metabolite pathways, targeted metabolomics, as well as metagenomics to better understand relationships between the brain, gut microbiome, and behavior in ASD. Future studies could also leverage Mendelian randomization (MR) analysis to evaluate potential causal relationships between stool metabolite levels and ASD. However, this approach is currently limited by the lack of adequate data. The only stool-based metabolite genome-wide association study (GWAS) available has a very small sample size and is not publicly accessible60. While serum-based GWAS data exist, they are constrained by relatively low sample sizes, which complicates MR analyses. Specifically, the limited number of genome-wide significant single nucleotide polymorphisms (SNPs) that are independent (i.e., not in linkage disequilibrium) poses challenges for their use as robust instrumental variables61. Despite these current limitations, we believe that this may be an important future technique to better assess causality. Finally, the current study focused on fecal metabolites due to their importance in impacting the brain via vagal signaling. However, given the fact that some tryptophan metabolites can pass the intestinal barrier and the blood–brain barrier (e.g., KYN, indoles), future studies should focus on serum metabolites for a more comprehensive picture of metabolite-brain-behavior associations. We note that a study by Yap and colleagues (2021) found that microbiome differences between ASD and NT samples were driven by less diverse diets, likely due to significantly restricted ASD food preferences, and thus cautioned against claiming a causative role of the microbiome in ASD pathophysiology62. However, in the current study, a post hoc analysis using independent samples t-tests found no group differences in metabolite concentrations between the observed diet types and no associations with diet between groups or significant correlations between diet and metabolites (Supplemental Fig. We cannot rule out the possibility of unreliable reporting of dietary patterns by ASD participants or their parents could be responsible for the lack of group differences. Future research should include more reliable and in-depth dietary analyses of participants (and their mothers during the prenatal stage), and use tools such as the foodMAST platform's foodomic analysis63 to circumvent issues with recording children's diet (e.g., parent recall bias, calculating accurate portion size)64. Nevertheless, the current study addresses many critiques of prior studies suggested by Yap and colleagues62. We included microbiome-relevant factors as covariates (age, sex, BMI), used multiple comparisons corrections in all GLM analyses, excluded participants on antibiotics, prebiotics, and probiotics, and measured and considered other factors potentially affecting the microbiome (sleep, delivery method, GI issues, medication usage). In summary, our study reveals that atypical activity in several brain regions previously implicated in ASD pathophysiology is associated with several tryptophan metabolites and symptomatology in youth with ASD. Further, the mediation analysis was consistent with a model that the fecal metabolite abundance is associated with brain activity in regions of interest, contributing to ASD symptomatology. Although our study design is unable to demonstrate causality, these findings represent an important step toward mechanistic integrated models of body-brain-behavior relationships in ASD, with potential implications for future interventions. Future studies incorporating longitudinal designs focusing on critical periods of pre- and postnatal development, as well as interventional designs, are needed to further explore these relationships and their relevance in ASD. Participants were recruited from healthcare clinics in Los Angeles, through advertising in the local community and social media, and by word-of-mouth. Inclusion criteria for all participants included: (a) aged 8–17 years old; (b) IQ of at least 79 on either Full-Scale Intelligence Quotient (FSIQ), or Verbal Comprehension Index (VCI) of the Wechsler Abbreviated Scale of Intelligence 2nd edition (WASI-II)65; and c) right-handed as assessed by a questionnaire adapted from Crovitz and Zener66. Exclusion criteria for all participants included: (a) history of head injury with loss of consciousness greater than 5 min; (b) not sufficiently fluent in English or parent who did not have English proficiency (as not all assessments have been validated in other languages); (c) born before 36 weeks of gestation; (d) contraindications to participating in MRI; (e) on probiotics/prebiotics for the past two weeks; and (f) on antibiotics in the past month. Additional inclusion criteria for the NT group were: (a) no first-degree relatives diagnosed with ASD; (b) a t-score < 65 on the Conners-3AI parent67 indicating no attention deficit hyperactivity disorder; (c) a t-score < 60 on the Social Responsiveness Scale 2nd edition (SRS-2)68 indicating low likelihood of ASD; and (d) no psychological or neurological disorders. For the ASD group, the Autism Diagnostic Observation Schedule (ADOS-2)69 and the Autism Diagnostic Interview-Revised (ADI-R)70 were administered by a research-certified assessor to confirm ASD diagnosis. Of the ASD participants, 9 were taking antidepressants, 1 was taking anticonvulsants, 11 were on stimulants, and 3 were taking antipsychotics at the time of participation (metabolites were adjusted for medication usage, see Methods subsection “Preprocessing of metabolomic data”). No NT participants were taking medication at time of participation. All participants were instructed to abstain from antibiotic usage for 30 days and probiotics for 14 days prior to participation. This study was approved by the University of Southern California's Institutional Review Board (Approval Number: UP-19-00522). Prior to their participation, all participants and parents/legally authorized representatives were emailed a copy of the informed consent and assent forms to give them adequate time to review the forms and ask questions. In person, the informed consent, assent forms, and study procedures were reviewed with the parents/legally authorized representatives and children prior to obtaining written informed consent and assent. Data were collected and stored on REDCap. The study took place over two days. Within 72 h prior to their second visit, participants collected a stool sample. On the second day, participants brought their stool sample into the lab and then participated in the fMRI sessions. In addition to the screening measures, parents completed the Sensory Experiences Questionnaire (SEQ-3)71 to assess sensory processing and the Screen for Child Anxiety Related Emotional Disorders (SCARED-P)72 to measure anxiety symptoms. Participants completed the Disgust Propensity and Sensitivity Scale-Revised (DPSS-R)73 to assess frequency of disgusting experiences and the emotional impact of disgusting stimuli, the Alexithymia Questionnaire for Children (AQC)74 to measure alexithymia, and the Body Perception Questionnaire-Short Form (BQP-SF)75 to measure interoception. We also collected data on variables that could impact the gut microbiome. The following variables were collected from the parent or the child including: birth delivery method, prenatal antibiotic usage, antibiotic and probiotic usage during infancy, gastrointestinal symptoms (Gastrointestinal Symptom Rating Scale)76, stool consistency (Bristol Stool Form Scale)77, sleep (Adolescent Sleep Wake Scale [ASWS]78; and Family Inventory of Sleep Habits [FISH])79, and current medication usage. For diet, parents were asked to report the type of diet that best reflects what their child consumes on a regular basis with the following options: Standard American (high consumption of processed foods, red meat, poultry, fish, eggs, dairy products, vegetables and fruits), Modified American (high consumption of whole grains, some processed foods, poultry, fish, eggs, dairy, vegetables and fruits), Mediterranean, Paleo, Vegetarian, Gluten Free, Dairy Free, Low FODMAP, or Other. No parents reported their child followed a Low FODMAP or Vegetarian diet. Standard American and Modified American were then grouped into the category “American” (high consumption of whole grains, some processed foods such as frozen and packaged foods as well as whole grain pasta and breads, limited quantities of poultry, fish, eggs and dairy, and vegetables and fruits are consumed in moderate to large quantities) and the Mediterranean, Paleo, Gluten Free, Dairy Free, and Other were grouped into the “Other” category. 2 for the diet breakdown by group. The ADOS-2 is a standardized, observational assessment to assess and diagnose autism spectrum disorders. Research reliable staff administered the ADOS-2 to children in the ASD group to verify ASD diagnosis. As the two modules have two different scoring algorithms, for data analysis we utilized the Module 3 algorithm to calculate comparable scores for participants that were assessed with Module 4. The ADI-R is a structured interview classically used for diagnosing ASD, planning treatment, and distinguishing ASD from other developmental disorders. The ADI-R has three domains: language/communication, reciprocal social interactions, and repetitive behaviors/interests. The AQC is a standardized, self-reported questionnaire for children aged 9-15 to assess three domains of alexithymia including difficulty describing feelings, difficulty identifying feelings, and externally oriented thinking. The difficulty describing feelings and difficulty identifying feelings scores were combined for a two-factor total. The externally oriented thinking sub-score was not used in this study due to its low Cronbach's alpha in children80. Self-reported sleep quality was measured with the ASWS. The ASWS has five dimensions: going to bed, falling asleep, maintaining sleep, reinitiating sleep, and returning to wakefulness. A full-scale sleep quality score is obtained by taking the mean of the subscales. The full-scale sleep quality score was used in this study. The BPQ-VSF is a self-reported measure of interoception. The very short form includes 12 items with the highest factor loadings to generate high fidelity scores from the original measure81. The Bristol Stool Form Scale is a visual scale of stool density to assess gastrointenstial transit time. The Conners 3AI-Parent is a parent reported measure of attention deficit hyperactivity disorder (ADHD) symptoms for children 6–18 years old. It was used as a screening measure for the NT group but not the ASD group, as ADHD is highly co-occurring with ASD82. The child version of the DPSS-R is a self-reported measure used to assess the frequency of disgust experiences (disgust propensity) and the negative emotional impact of disgusting stimuli (disgust sensitivity). The FISH is a parent-completed questionnaire to assess sleep habits in children. The scale includes questions related to daytime routine, pre-bedtime habits, sleep environment, bedtime routine, and parental habits. The total score was used in this study as an overall measure of sleep habits. The GSRS is a self-reported instrument to assess the presence of gastrointestinal symptoms. The SCARED-P is a parent reported measure of anxiety symptoms in children aged 8–18 years old. It has five subscales: separation anxiety, generalized anxiety, social anxiety, panic or somatic symptoms, and school avoidance, which can be combined to obtain a total anxiety score. The SEQ is a parent-reported measure of sensory response patterns (hypersensitivity, hyposensitivity, enhanced perception, and sensory interests, repetitions, and seeking), sensory modalities (tactile, auditory, gustatory, auditory, vestibular/proprioception), and sensory contexts (non-social and social). The hypersensitivity, hyposensitivity, enhanced perception, non-social, social, and vestibular/proprioception were used as variables of interest in this study due to their hypothesized relationships with the fMRI tasks and/or metabolites. The SRS-2 is a parent reported questionnaire for children aged 4–18 years that is used as both a screening tool and to aid in clinical diagnosis of ASD. The measure includes questions related to restrictive interests, repetitive behaviors, deficits in social interactions, and social communication. The WASI-II is a standardized measure of intelligence for ages 6–90. There are four subtests: block design, vocabulary, matrix reasoning, and similarities. Block design and matrix reasoning are combined to obtain a perceptual reasoning index (PRI) and vocabulary and similarities are combined for a verbal comprehension index (VCI). The full-scale intelligence quotient (FSIQ) consists of all four subtests. The WASI-II was administered by trained research staff. After completion of their day 1 visit, participants were given a stool collection kit (specimen cup, wooden spatula, plastic bag, Fisherbrand Scientific Commode Specimen Collection System, gloves, ice packs, and an insulated transportation container). They were instructed to collect a stool sample within 72 h prior to their MRI, freeze the sample at home, and transport it in the insulated transportation container with ice packs to the lab. Once in the lab, the sample was placed in a −80 °C degree freezer for storage (first at USC, and then at UCLA where they were aliquoted under liquid nitrogen and stored in a −80 °C degree freezer). Aliquoted samples were shipped on dry ice with a stool collection log to Metabolon Inc. for further processing and analysis on their global metabolomics and bioinformatics platform (Metabolon, 617 Davis Drive, Durham, NC). See the section “Metabolite analysis (All information in this section provided by Metabolon, Inc)” for more details on sample analysis. MRI data were acquired on a 3 Tesla MAGNETOM Prisma (Siemens, Erlangen, Germany) with a 20-channel head coil. A 5-min structural T1-weighted MPRAGE was acquired for each participant (TR = 1950 ms, TE = 3.09 ms, flip angle = 10°, 256 × 256 matrix, 176 sagittal slices, 1 mm isotropic resolution). Each functional scan consisted of an echo-planar imaging (EPI; 150 whole-brain volumes) acquired with the following parameters: TR = 2 s, TE = 30 ms, flip angle = 90°, 64 × 64 matrix, in-plane resolution 2.5 × 2.5 mm, and 41 transverse slices, each 2.5 mm thick, covering the whole-brain with a multiband factor of three. Spin Echo EPI field mapping data was also acquired in AP and PA directions with identical geometry to the EPI data for EPI off-resonance distortion correction (TR = 1020 ms, TE1 = 10 ms, TE2 = 12.46 ms, flip angle = 90°, FOV = 224 × 224 × 191 mm3, voxel size = 2.5 mm isotropic). All participants completed a practice MRI session in a mock MRI scanner prior to the fMRI tasks to become familiarized with the task and the MRI environment and to increase comfortability and minimize head motion. Functional MRI procedure, task stimuli, fMRI acquisition, and data preprocessing were completed following the protocol previously published in Kilroy et al.38. We utilized a head-motion cut-off of absolute FD > 1.5 mm. Five participants (4 ASD, 1 NT) were excluded for head motion in the watching facial expressions and hand actions task, 3 (2 ASD, 1 NT) for disgust processing, and 2 (1 ASD, 1 NT) from watching others being touched. There were no significant differences in relative head motion between the two groups for the disgust processing (t = 0.981, p = 0.33) and watching others being touched (t = −1.029, p = 0.307) tasks, but significant differences were present for the observation of facial expressions and hand actions task (t = −2.572, p = 0.015). Please see section “fMRI processing” for details regarding motion correction. The task-based fMRI paradigms were selected based on existing literature showing significant ASD vs. NT differences during these tasks, their relevance to key ASD symptomatology (socio-emotional processing and sensory sensitivities)38,46,83,84 and/or their relevance to vagally mediated emotional processes (disgust and emotion processing)49. The fMRI tasks included: watching videos of facial expressions/body actions, physical and social disgust processing tasks, and watching videos of others being touched (Supplemental Fig. Stimuli were presented using the Psychophysics Toolbox85 on MATLAB. During all tasks, participants were instructed to simply watch all videos and remain as still as possible. fMRI tasks are described in A–C below. Watching videos of facial expressions and hand actions (n = 78; 38 NT [19 female, 19 male], 40 ASD [11 female, 29 male]): One 9-min run with five 15-s blocks of video-stimuli were shown. 1A shows, blocks consisted of one of three categories of stimuli: emotional facial expressions (e.g., happy expression), non-emotional facial expressions (e.g., tongue to lip), or bimanual hand actions (e.g., playing the xylophone). Each video was presented for 3.75 s followed by a 1.25 s black screen between each stimulus, there were 3 videos per block, and both male and female actors were included in each block. For further details on stimuli, please see Kilroy et al.38. Disgust processing (n = 46; 22 NT [12 female, 10 male], 24 ASD [6 female, 18 male]): There were four categories of stimuli, disgusting foods, disgusted facial expressions, neutral foods, and neutral facial expressions (Supplemental Fig. The neutral and the disgusted facial expressions were chosen from an online repository (NimStim)86 and from previous research (EmStim)38 then edited and counterbalanced so that each participant saw the same actor displaying a neutral and disgusted facial expression. To ensure that the neutral food images were indeed items the participant truly had no preferential or disgusting feelings for, all participants were administered a questionnaire prior to participating in the study, to assess their preferences for each food stimuli. One fMRI run was presented to all participants, including six blocks per stimulus category. Within each 15-s block, three different images from the same category were presented with a 250-ms fixation crosshair between each stimulus (e.g., three different disgusting food images). Thus, the fMRI task consisted of 24 blocks (5 per stimulus category), lasting for a single 10-min run. C. Watching others being touched (n = 37; 19 NT [11 female, 8 male], 18 ASD [5 female, 13 male]): Participants watched four different videos where a person strokes the arm of another person in the MRI scanner with: 1) their hand with glove on (social touch), 2) a dry sponge (object touch), 3) their hand with glove on hovering next to the person's arm (social touch control), and 4) a dry sponge hovering next to the person's arm (object touch control) following a similar to the protocol used in Green et al. 2015 (Supplemental Fig. During the rest blocks, participants were shown a black crosshair in the middle of a white screen. Excluding an initial junk block, five blocks of each stimulus condition were alternated with rest in a pseudo-random sequence. Stimuli conditions were counterbalanced across participants. We note that our original intention was to physically touch participants while in the scanner, but as this task was largely conducted during the initial period of the COVID-19 pandemic, we were obliged to remain at a 6-ft distance from our participants, and thus used videos of touch instead, as this has previously been shown to show strong effects88,89. Independent samples t-tests and Fisher's exact tests were conducted using SPSS Statistics (Version 29.0.2.0) to determine ASD-NT differences in demographic and behavioral variables. Significance was set at p < 0.05. Instrument and Process Validity: Instrument variability was determined by calculating the median relative standard deviation (RSD) for the internal standards that were added to each sample prior to injection into the mass spectrometers. Overall process variability was determined by calculating the median RSD for all endogenous metabolites (i.e., non-instrument standards) present in 100% of the Client Matrix samples, which are technical replicates of pooled client samples. Values for instrument and process variability met Metabolon's acceptance criteria. Following receipt, samples were inventoried and immediately stored at −80 °C. Each sample received was accessioned into the Metabolon LIMS system and was assigned by the LIMS a unique identifier that was associated with the original source identifier only. This identifier was used to track all sample handling, tasks, results, etc. The samples (and all derived aliquots) were tracked by the LIMS system. All portions of any sample were automatically assigned their own unique identifiers by the LIMS when a new task was created; the relationship of these samples was also tracked. All samples were maintained at −80 °C until processed. Samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. Several types of controls were analyzed in concert with the experimental samples: a pooled matrix sample generated by taking a small volume of each experimental sample (or alternatively, use of a pool of well-characterized human plasma) served as a technical replicate throughout the data set; extracted water samples served as process blanks; and a cocktail of QC standards that were carefully chosen not to interfere with the measurement of endogenous compounds were spiked into every analyzed sample, allowed instrument performance monitoring and aided chromatographic alignment. Instrument variability was determined by calculating the median relative standard deviation (RSD) for the standards that were added to each sample prior to injection into the mass spectrometers. Overall process variability was determined by calculating the median RSD for all endogenous metabolites (i.e., non-instrument standards) present in 100% of the pooled matrix samples. Experimental samples were randomized across the platform run with QC samples spaced evenly among the injections. All methods utilized a Waters ACQUITY ultra-performance liquid chromatography (UPLC) and a Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer interfaced with a heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution. The sample extract was dried then reconstituted in solvents compatible to each of the four methods. Each reconstitution solvent contained a series of standards at fixed concentrations to ensure injection and chromatographic consistency. One aliquot was analyzed using acidic positive ion conditions, chromatographically optimized for more hydrophilic compounds. In this method, the extract was gradient eluted from a C18 column (Waters UPLC BEH C18-2.1 × 100 mm, 1.7 µm) using water and methanol, containing 0.05% perfluoropentanoic acid (PFPA) and 0.1% formic acid (FA). Another aliquot was also analyzed using acidic positive ion conditions; however, it was chromatographically optimized for more hydrophobic compounds. In this method, the extract was gradient eluted from the same aforementioned C18 column using methanol, acetonitrile, water, 0.05% PFPA and 0.01% FA and was operated at an overall higher organic content. Another aliquot was analyzed using basic negative ion optimized conditions using a separate dedicated C18 column. The basic extracts were gradient eluted from the column using methanol and water, however, with 6.5 mM Ammonium Bicarbonate at pH 8. The fourth aliquot was analyzed via negative ionization following elution from a HILIC column (Waters UPLC BEH Amide 2.1 × 150 mm, 1.7 µm) using a gradient consisting of water and acetonitrile with 10 mM Ammonium Formate, pH 10.8. The MS analysis alternated between MS and data-dependent MSn scans using dynamic exclusion. The scan range varied slighted between methods but covered 70–1000 m/z. Raw data files are archived and extracted as described below. The informatics system consisted of four major components, the Laboratory Information Management System (LIMS), the data extraction and peak-identification software, data processing tools for QC and compound identification, and a collection of information interpretation and visualization tools for use by data analysts. The hardware and software foundations for these informatics components were the LAN backbone, and a database server running Oracle 10.2.0.1 Enterprise Edition. The purpose of the Metabolon LIMS system was to enable fully auditable laboratory automation through a secure, easy to use, and highly specialized system. The scope of the Metabolon LIMS system encompasses sample accessioning, sample preparation and instrumental analysis and reporting and advanced data analysis. All the subsequent software systems are grounded in the LIMS data structures. It has been modified to leverage and interface with the in-house information extraction and data visualization systems, as well as third-party instrumentation and data analysis software. Raw data was extracted, peak-identified and QC processed using Metabolon's hardware and software. These systems are built on a web-service platform utilizing Microsoft's.NET technologies, which run on high-performance application servers and fiber-channel storage arrays in clusters to provide active failover and load-balancing. Compounds were identified by comparison to library entries of purified standards or recurrent unknown entities. Metabolon maintains a library based on authenticated standards that contains the retention time/index (RI), mass to charge ratio (m/z), and chromatographic data (including MS/MS spectral data) on all molecules present in the library. Furthermore, biochemical identifications are based on three criteria: retention index within a narrow RI window of the proposed identification, accurate mass match to the library +/−10 ppm, and the MS/MS forward and reverse scores between the experimental data and authentic standards. While there may be similarities between these molecules based on one of these factors, the use of all three data points can be utilized to distinguish and differentiate biochemicals. More than 3300 commercially available purified standard compounds have been acquired and registered into LIMS for analysis on all platforms for determination of their analytical characteristics. Additional mass spectral entries have been created for structurally unnamed biochemicals, which have been identified by virtue of their recurrent nature (both chromatographic and mass spectral). These compounds have the potential to be identified by future acquisition of a matching purified standard or by classical structural analysis. A variety of curation procedures were carried out to ensure that a high-quality data set was made available for statistical analysis and data interpretation. The QC and curation processes were designed to ensure accurate and consistent identification of true chemical entities, and to remove those representing system artifacts, mis-assignments, and background noise. Metabolon data analysts use proprietary visualization and interpretation software to confirm the consistency of peak identification among the various samples. Library matches for each compound were checked for each sample and corrected if necessary. Peaks were quantified using area-under-the-curve. Peak area values were log transformed and KNN imputation was applied for missing data90. Next, data was Z score normalized and adjusted for use of antidepressants, vitamins, supplements, laxatives, antihistamines, stimulants, cognition enhancers, and antipsychotics. Specifically we regressed out significant medication/supplement effects identified using a backward selection approach (function “MASS::stepAIC” in R using the BIC, i.e., log(n) degrees of freedom). The adjusted features were then used in downstream analyses. A priori metabolite targets of interest included 26 named metabolites in the tryptophan pathway (see Fig. Contrast analysis was applied within the framework of the general linear model (GLM) to determine ASD-NT differences in 26 tryptophan metabolites. The model included group as a factor, and sex, age, and BMI as covariates. As children with ASD in our sample had significantly higher levels of gastrointestinal symptoms compared to the NT children (p < 0.001; Supplemental Table 1) we also ran GLMs controlling for gastrointestinal symptoms (GSRS) and diet to assess potential confounding effects of GI symptoms on the levels of tryptophan-related metabolites. Significance was considered at a false discovery rate of q < 0.05. Cohen's d was calculated to provide an effect size for differences. R Version 4.2.1 was used for all GLM analysis. All analyses followed best practices in fMRI analysis, as detailed in our prior studies38,49. The data analytic approach used to address each of our research questions utilized FMRIB's Software Library 6.0 (FSL)91,92,93,94,95. Standard preprocessing pipeline was performed involving: (a) structural T1 brain extraction and non-brain tissue removal; (b) smoothing with 5 mm FWHM Gaussian kernel; (c) B0 unwarping along y-axis; (d) high pass filter with 100 s cutoff; (e) realignment using MCFLIRT to obtain motion estimates; and (f) Independent component analysis (ICA). Preprocessed data was fed into the ICA AROMA algorithm96, which filtered out noise and motion components from the whole brain signal. Registration to the MNI-152 standard atlas using 12 degrees-of-freedom affine transformation and FNIRT nonlinear registration were performed92,93. Individual participants' statistical images were subjected to higher-level mixed-effects analyses using FSL's FLAME Stage 1 algorithm, modeling the stimulus conditions for each participant as separate regressors. For watching facial expressions and hand actions, regressors included: emotional faces, non-emotional faces, and bimanual hand actions. For disgust processing, regressors included: disgusting foods, neutral foods, disgusted facial expressions, neutral facial expressions. Subject-specific head motion parameters were used as nuisance regressors. For observation of others being touched, regressors included social touch and object touch. Between-group comparisons between the NT and ASD groups were performed using higher level mixed-effects analyses with FSL's FLAME 1 algorithm. Age, Sex, and IQ were used as covariates in all group-level analyses. For disgust processing, groups were contrasted on: disgusting foods>rest, disgusted faces>rest. For observation of others being touched, groups were contrasted on: social touch>rest and object touch>rest. For the facial expressions/hand action task and the disgust tasks, the resulting group-level images for all models were thresholded at voxel Z > 3.1, with a cluster size probability correction threshold of p < 0.05. For observation of others being touched, a more lenient threshold (Z  >  2.3 cluster size probability threshold of p < 0.05) was used to have more sensitivity to detect effects given the more subtle observation (rather than physical touch) task used, due to COVID-19 restrictions (see Methods: fMRI Tasks, Watching others being touched). In addition, for disgust and facial expression/hand action tasks, for hypothesized regions of interest (ROIs), a small volume correction (SVC) analysis with a significance threshold of p < 0.01 using predefined masks for disgust and observation tasks. For the facial expression/hand action task, we used structurally defined anterior insula parcellations from extant literature and the Harvard-Oxford atlas parcellations for the pACC and amygdala. For the IFGop, we used a hand-drawn anatomically derived ROI38 and previously published insula parcellations97. For the disgust task, ROIs for SVC analysis were defined utilizing the Neurosynth database (which performs automated large-scale meta-analyses of fMRI data), using the search terms: “disgust”, “emotional faces”, and “food”, and we also included insula parcellations from extant literature97. Functional ROIs were then masked with structural ROIs from the Harvard-Oxford atlas (thresholded at 30% probability) to ensure they captured non-overlapping regions. GLMs were applied within the ASD and NT groups to test brain-behavior, brain-metabolite, and metabolite-behavior relationships. The GLMs included group as a factor, and sex, age, IQ, and BMI were included as covariates. As a measure of effect size, we report the standardized beta (Std β). Brain ROIs were chosen based on group differences in fMRI tasks as well as prior studies supporting atypicalities in brain activity in the chosen ROIs38,46,49,83,84. The Benjamini-Hochberg method to correct for multiple comparisons was used; the false discovery reporting threshold set at 10% (FDR)99. We used FDR correction for the number of dependent variables in each analyses. Specifically for brain-metabolite and brain-behavior analyses, FDR correction was for the number of ROIs compared. For metabolite-behavior analyses, FDR correction was for the number of metabolites compared. To limit the number of comparisons, only metabolites that significantly correlated with brain activity were included in the metabolite-behavior analyses. As a post hoc analysis, we ran GLMs with all primary variables and all tryptophan metabolites (see Supplemental Table 7). The GLM model included group as a factor, and sex, age, IQ, and BMI were included as covariates. Exploratory mediation analyses were conducted to determine if the brain regions that differed between ASD and NT statistically mediated the relationships between metabolites and behavior in the ASD group. The variables included in the mediation models were selected based on ROIs that had both significant ROI-metabolite associations and significant ROI-behavior associations. Mediation modeling was performed using lavaan in R. We estimated the bootstrapped 95% percentile confidence intervals for the indirect effects100. Confidence intervals that do not contain zero are considered significant. Because age, sex, and BMI are collinear, we ran analyses using only age and FSIQ as covariates. 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M. et al. Advances in functional and structural MR image analysis and implementation as FSL. Woolrich, M. W., Ripley, B. D., Brady, M. & Smith, S. M. Temporal autocorrelation in univariate linear modeling of FMRI data. Pruim, R. H. R. et al. ICA-AROMA: a robust ICA-based strategy for removing motion artifacts from fMRI data. Three systems of insular functional connectivity identified with cluster analysis. Nieminen, P. Application of standardized regression coefficient in meta-analysis. Controlling the false discovery rate: a practical and powerful approach to multiple testing. Confidence limits for the indirect effect: distribution of the product and resampling methods. We thank all our participants, research assistants, and the Integrative Biostatistics and Bioinformatics Core at the Goodman Luskin Microbiome Center for their contributions to this study. We also thank Ruty Mehrian-Shai, Antonio Damasio, Sarkis Mazmanian, and Jonas Kaplan for helpful discussions on this study. This work was funded by Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD079432; PI: L.A.Z., M.D.) Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the NIH or the Department of Defense. Additional support was provided by the Nedra Gillette Endowed Research Fellowship E.K. These authors contributed equally: Jennifer S. Labus, Emeran A. Mayer. Chan Division of Occupational Science and Occupational Therapy, University of Southern California, Los Angeles, CA, USA Lisa Aziz-Zadeh, Sofronia M. Ringold, Aditya Jayashankar, Emily Kilroy & Christiana Butera Brain and Creativity Institute, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA, USA Lisa Aziz-Zadeh, Sofronia M. Ringold, Aditya Jayashankar, Emily Kilroy & Christiana Butera Division of Biokinesiology and Physical Therapy, University of Southern California, Los Angeles, CA, USA Oppenheimer Center for the Neurobiology of Stress and Resilience, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA Jonathan P. Jacobs, Skylar Tanartkit, Swapna Mahurkar-Joshi, Jennifer S. Labus & Emeran A. Mayer Jonathan P. Jacobs, Skylar Tanartkit, Swapna Mahurkar-Joshi, Jennifer S. Labus & Emeran A. Mayer Goodman-Luskin Microbiome Center, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA Jonathan P. Jacobs, Skylar Tanartkit, Swapna Mahurkar-Joshi, Jennifer S. Labus & Emeran A. Mayer Imaging Genetics Center, Mark and Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Marina del Rey, CA, USA Department of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, USA Ahmanson-Lovelace Brain Mapping Center, University of California Los Angeles, Los Angeles, CA, USA You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar You can also search for this author inPubMed Google Scholar ; First draft of manuscript: L.A.Z., S.M.R., J.S.L., and E.A.M. 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